Assigning the phenotype of a natural regulatory T-cell to the human T-cell line, KARPAS-299

Int J Mol Med. 2006 Feb;17(2):275-8.

Abstract

The human CD30-positive anaplastic large (T-) cell lymphoma cell line, KARPAS-299 (DSM ACC31), was established from blast cells in the peripheral blood from a case of non-Hodgkin lymphoma in 1988. We describe the mRNA and surface expression in KARPAS-299 cells of a panel of markers highly restricted to human natural regulatory T-cells and associated with their suppressive activity, including FOXP3, CD25, IL-10, TGF-beta1, CD62L, and Lag-3. Results obtained from co-culturing human peripheral blood leukocytes with KARPAS-299 cells assigned a suppressive phenotype to the latter ones. In conclusion, KARPAS-299 cells show characteristics typical of natural regulatory T-cells and, thus, represent a valuable model for studying regulatory T-cell function, which may also facilitate drug development aimed at the modulation of regulatory T-cell activity for the pharmacological therapy of, for example, autoimmune diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Coculture Techniques
  • Forkhead Transcription Factors / metabolism
  • Humans
  • Phenotype
  • RNA, Messenger / genetics
  • T-Lymphocytes, Regulatory / metabolism*
  • Transforming Growth Factor beta / metabolism
  • Transforming Growth Factor beta1

Substances

  • FOXP3 protein, human
  • Forkhead Transcription Factors
  • RNA, Messenger
  • TGFB1 protein, human
  • Transforming Growth Factor beta
  • Transforming Growth Factor beta1