Induction of neurite outgrowth by (-)-(7R, 8S)-dihydrodehydrodiconiferyl alcohol from PC12 cells

Arch Pharm Res. 2005 Dec;28(12):1337-40. doi: 10.1007/BF02977898.

Abstract

A lignan derivative, (-)-(7R, 8S)-dihydrodehydrodiconiferyl alcohol (DHDA), was isolated from Kalopanax septemlobus L. and was observed to have neuritogenic activity. DHDA at 50 microM caused a marked induction of neurite outgrowth and an enhancement of nerve growth factor (NGF)-mediated neurite outgrowth from PC12 cells. However, it did not exhibit any neurotrophic action. At 50 microM, DHDA enhanced NGF-induced neurite-bearing activity. This activity was partially blocked by the mitogen-activated protein kinase (MAPK) inhibitor PD98059 and by GF109203X, a protein kinase C (PKC) inhibitor. These results suggest that DHDA can induce neurite outgrowth and enhance NGF-induced neurite outgrowth from PC12 cells by amplifying up-stream steps such as MAPK and PKC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Araliaceae
  • Dose-Response Relationship, Drug
  • Enzyme Inhibitors / pharmacology
  • Flavonoids / pharmacology
  • Indoles / pharmacology
  • Lignin / analogs & derivatives*
  • Lignin / antagonists & inhibitors
  • Lignin / isolation & purification
  • Lignin / pharmacology
  • MAP Kinase Signaling System / drug effects
  • Maleimides / pharmacology
  • Medicine, East Asian Traditional
  • Mitogen-Activated Protein Kinase Kinases / antagonists & inhibitors
  • Nerve Growth Factor / pharmacology
  • Neurites / drug effects*
  • Neurites / physiology
  • PC12 Cells
  • Plant Bark / chemistry
  • Plant Stems
  • Plants, Medicinal
  • Protein Kinase C / antagonists & inhibitors
  • Rats

Substances

  • Enzyme Inhibitors
  • Flavonoids
  • Indoles
  • Maleimides
  • pinonesinol
  • Lignin
  • Nerve Growth Factor
  • Protein Kinase C
  • Mitogen-Activated Protein Kinase Kinases
  • bisindolylmaleimide I
  • 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one