Gene-centromere linkage mapping by PCR analysis of individual oocytes

Genomics. 1992 Jul;13(3):713-7. doi: 10.1016/0888-7543(92)90146-j.

Abstract

We describe a general method of determining the recombination fraction between a polymorphic locus and the centromere in any species where single oocytes can be obtained. After removal of the first polar body, each oocyte is analyzed by PCR. The frequency of oocytes heterozygous at the polymorphic locus is used to estimate the recombination fraction. We estimate a recombination fraction of 0.15 between the mouse major histocompatibility complex (H-2) and the centromere of chromosome 17.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Centromere
  • Chromosome Mapping / methods*
  • DNA / genetics
  • Female
  • Genetic Linkage*
  • H-2 Antigens / genetics
  • Heterozygote
  • Mice
  • Mice, Inbred Strains
  • Molecular Sequence Data
  • Oocytes*
  • Polymerase Chain Reaction
  • Recombination, Genetic

Substances

  • H-2 Antigens
  • DNA