A new animal model of choroidal neovascularization

Acta Ophthalmol Scand. 2005 Dec;83(6):697-704. doi: 10.1111/j.1600-0420.2005.00566.x.


Purpose: The purpose of this study was to evaluate the ability of different methods to induce choroidal neovascularization (CNV) in the domestic pig.

Methods: A total of 26 Danish landrace pigs was used. A sample of 22 eyes in 12 pigs underwent retinal photocoagulation with a xenon lamp, six eyes in four pigs underwent retinal photocoagulation with a diode laser, and mechanical rupture of Bruch's membrane (BM) was induced in 12 pigs following surgical debridement of the retinal pigment epithelium without damage to the neuroretina.

Results: All 12 pigs (100%) in the group with mechanical rupture of BM developed CNV. The induced membranes were morphologically similar to CNV membranes in humans. Induced CNV was found in 13 of 22 (54%) xenon lamp-treated animals and in five of six (83%) diode laser-treated animals. The CNV in these groups was small and the morphology of the induced lesions was dominated by retinal gliosis and retinal neovascularization, probably due to a marked destruction of the neuroretina.

Conclusions: Surgical debridement of the retinal pigment epithelium followed by mechanical rupture of BM is a reproducible method of producing CNV in the domestic pig, whereas photocoagulation gives rise to glially derived subretinal fibrovascular membranes and primarily retinal neovascularization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bruch Membrane / injuries
  • Choroid / blood supply
  • Choroidal Neovascularization / etiology
  • Choroidal Neovascularization / metabolism
  • Choroidal Neovascularization / pathology*
  • Debridement / methods
  • Disease Models, Animal*
  • Factor VIII / metabolism
  • Laser Coagulation / methods
  • Reproducibility of Results
  • Rupture
  • Swine*
  • von Willebrand Factor / metabolism


  • von Willebrand Factor
  • Factor VIII