Fast chromatin immunoprecipitation assay

Nucleic Acids Res. 2006 Jan 5;34(1):e2. doi: 10.1093/nar/gnj004.


Chromatin immunoprecipitation (ChIP) is a widely used method to explore in vivo interactions between proteins and DNA. The ChIP assay takes several days to complete, involves several tube transfers and uses either phenol-chlorophorm or spin columns to purify DNA. The traditional ChIP method becomes a challenge when handling multiple samples. We have developed an efficient and rapid Chelex resin-based ChIP procedure that dramatically reduces time of the assay and uses only a single tube to isolate PCR-ready DNA. This method greatly facilitates the probing of chromatin changes over many time points with several antibodies in one experiment.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Animals
  • Cells, Cultured
  • Chromatin / chemistry*
  • Chromatin Immunoprecipitation / methods*
  • DNA / analysis
  • DNA / isolation & purification*
  • Rats
  • Reproducibility of Results
  • Saccharomyces cerevisiae / genetics
  • Time Factors


  • Chromatin
  • DNA