Molecular cloning of canine mucosal addressin cell adhesion molecule-1 cDNA and its expression in normal tissues

J Vet Med Sci. 2005 Dec;67(12):1257-60. doi: 10.1292/jvms.67.1257.

Abstract

A cDNA encoding canine mucosal addressin cell adhesion molecule-1 (MAdCAM-1) was cloned. The entire open reading frame of canine MAdCAM-1 cDNA comprises 1137 bp, corresponding to 378 amino acid residues. The deduced amino acid sequence of canine MAdCAM-1 was 55.2%, 53.7%, and 52.4% identical to rat, mouse, and human MAdCAM-1, respectively. Canine MAdCAM-1 appeared to contain two immunoglobulin-like domains at the N-terminus, followed by a mucin-like domain and a third immunoglobulin-like domain. The structures of the dog, rat, and mouse proteins are likely similar because all of the cysteine residues in the immunoglobulin-like domains were conserved. Canine MAdCAM-1 mRNA was confirmed to express extremely in the mesenteric lymph node by RT-PCR.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Adhesion Molecules / genetics*
  • Cell Adhesion Molecules / metabolism
  • Cloning, Molecular
  • DNA Primers
  • DNA, Complementary / genetics
  • Dogs / genetics*
  • Dogs / metabolism
  • Immunoglobulins / genetics*
  • Immunoglobulins / metabolism
  • Molecular Sequence Data
  • Mucoproteins / genetics*
  • Mucoproteins / metabolism
  • Sequence Analysis, DNA
  • Sequence Homology

Substances

  • Cell Adhesion Molecules
  • DNA Primers
  • DNA, Complementary
  • Immunoglobulins
  • Mucoproteins