Abstract
The anti-tumor potential of components from Chinese herbal medicines has been greatly concerned. Alisol B acetate, a triterpene from Alismatis rhizoma, induced apoptotic cell death in human hormone-resistant prostate cancer PC-3 cells in a time- and concentration-dependent manner. A good correlation between loss of mitochondrial membrane potential and apoptotic cell death was apparent indicating the participation of mitochondria-related mechanism. Alisol B acetate induced Bax up-regulation and nuclear translocation; it also induced the activation of initiator caspase-8 and caspase-9, and executor caspase-3, suggesting the involvement of both extrinsic and intrinsic apoptosis pathways. Taken together, it is suggested that alisol B acetate induces apoptosis in PC-3 cells via a mitochondria-mediated mechanism with activation of caspase-8, -9 and -3. Furthermore, the Bax activation and translocation from the cytosol to nucleus might be a crucial response to the apoptotic effect.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alismataceae / chemistry*
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Antineoplastic Agents / pharmacology
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Antineoplastic Agents, Phytogenic / pharmacology
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Apoptosis / drug effects*
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Blotting, Western
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Caspases / metabolism
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Cell Nucleus / metabolism*
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Cholestenones / pharmacology*
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Curcumin / pharmacology
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Drug Resistance, Neoplasm
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Flow Cytometry
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Humans
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Male
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Membrane Potentials / drug effects
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Mitochondria / drug effects
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Neoplasms, Hormone-Dependent / drug therapy*
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Neoplasms, Hormone-Dependent / metabolism
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Neoplasms, Hormone-Dependent / pathology
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Nitric Oxide Synthase / antagonists & inhibitors
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Paclitaxel / pharmacology
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Prostatic Neoplasms / drug therapy*
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Prostatic Neoplasms / metabolism
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Prostatic Neoplasms / pathology
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Protein Transport / drug effects
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Proto-Oncogene Proteins c-bcl-2 / metabolism
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Triterpenes / pharmacology
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Tumor Cells, Cultured / drug effects
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bcl-2-Associated X Protein / metabolism*
Substances
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Antineoplastic Agents
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Antineoplastic Agents, Phytogenic
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Cholestenones
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Proto-Oncogene Proteins c-bcl-2
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Triterpenes
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alisol B monoacetate
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bcl-2-Associated X Protein
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Nitric Oxide Synthase
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Caspases
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Curcumin
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Paclitaxel