Analysis of tubulin isoforms by two-dimensional gel electrophoresis using SDS-polyacrylamide gel electrophoresis in the first dimension

J Biochem Biophys Methods. 1992 Jun;24(3-4):195-203. doi: 10.1016/0165-022x(94)90072-8.

Abstract

A two-dimensional electrophoretic system using SDS-polyacrylamide gel electrophoresis (SDS-PAGE) in the first dimension and isoelectric focusing (IEF) in the second dimension was devised. In spite of its simplicity, this method could show a markedly high resolution for tubulin isoforms and moreover could classify them into alpha- or beta-tubulin as a two-dimensional profile. With this method, seven alpha- and four beta-tubulin isoforms could be detected within axoneme from Tetrahymena cilia. Moreover this method could also resolve tubulin isoforms from the rabbit brain. These results indicate that the present two-dimensional gel electrophoresis is a useful tool for the electrophoretic analysis of tubulin isoforms from various sources.

MeSH terms

  • Animals
  • Brain Chemistry / physiology
  • Cilia / chemistry
  • Electrophoresis, Gel, Two-Dimensional*
  • Isoelectric Focusing*
  • Rabbits
  • Tetrahymena / chemistry
  • Tetrahymena / ultrastructure
  • Tubulin / analysis*
  • Tubulin / chemistry

Substances

  • Tubulin