Fish-isolated strains of Hartmannella vermiformis page, 1967: morphology, phylogeny and molecular diagnosis of the species in tissue lesions

Folia Parasitol (Praha). 2005 Nov;52(4):295-303.

Abstract

Based on morphological and molecular characterisation, four amoeba strains isolated from organs of freshwater fish were identified as Hartmannella vermiformis Page, 1967. Small subunit rRNA gene sequences of these strains expand the set of corresponding complete and almost complete sequences of this species to twelve. A new species-specific oligonucleotide probe inferred from recently available SSU rRNA gene sequences was designed and successfully tested in tissue lesions produced by one strain of H. vermiformis in experimentally infected fish.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amebiasis / diagnosis
  • Amebiasis / parasitology
  • Amebiasis / veterinary*
  • Animals
  • Base Sequence / genetics
  • Fish Diseases / diagnosis
  • Fish Diseases / parasitology*
  • Fishes
  • Genes, rRNA / genetics
  • Hartmannella / classification
  • Hartmannella / genetics*
  • Hartmannella / isolation & purification
  • Hartmannella / ultrastructure*
  • In Situ Hybridization, Fluorescence / methods
  • Likelihood Functions
  • Microscopy, Electron / methods
  • Molecular Sequence Data
  • Phylogeny*
  • Sequence Analysis, DNA

Associated data

  • GENBANK/DQ084363
  • GENBANK/DQ084364
  • GENBANK/DQ084365
  • GENBANK/DQ084366