High-throughput assay for small molecules that modulate zebrafish embryonic heart rate

Nat Chem Biol. 2005 Oct;1(5):263-4. doi: 10.1038/nchembio732. Epub 2005 Sep 18.


To increase the facility and throughput of scoring phenotypic traits in embryonic zebrafish, we developed an automated micro-well assay for heart rate using automated fluorescence microscopy of transgenic embryos expressing green fluorescent protein in myocardium. The assay measures heart rates efficiently and accurately over a large linear dynamic range, and it rapidly characterizes dose dependence and kinetics of small molecule-induced changes in heart rate. This is the first high-throughput micro-well assay for organ function in an intact vertebrate.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Biological Assay / methods*
  • Cardiovascular Agents / toxicity*
  • Dose-Response Relationship, Drug
  • Drug Evaluation, Preclinical / methods
  • Embryo, Nonmammalian / physiology
  • Feasibility Studies
  • Green Fluorescent Proteins / analysis*
  • Heart / drug effects*
  • Heart / embryology
  • Heart / physiology*
  • Heart Rate / drug effects*
  • Heart Rate / physiology
  • Myocardium / metabolism
  • Time Factors
  • Zebrafish


  • Cardiovascular Agents
  • Green Fluorescent Proteins

Associated data

  • PubChem-Substance/4266412
  • PubChem-Substance/4266413
  • PubChem-Substance/4266415
  • PubChem-Substance/4266416
  • PubChem-Substance/4266417