Ferrioxamine uptake in Yersinia enterocolitica: characterization of the receptor protein FoxA

Mol Microbiol. 1992 May;6(10):1309-21. doi: 10.1111/j.1365-2958.1992.tb00852.x.

Abstract

The gene for the high-affinity outer membrane ferrioxamine receptor FoxA of Yersinia enterocolitica was cloned in Escherichia coli K-12. A foxA mutant of Yersinia could be complemented by the cloned DNA fragment. The FoxA encoding region was sequenced and an open reading frame encoding 710 amino acids, including a signal sequence of 26 amino acids, was deduced. The mature FoxA protein consisted of 684 amino acids and had a molecular mass of 75,768 Da. FoxA shared 33% amino acid sequence homology with FhuA, the ferrichrome receptor of Escherichia coli. Based on the homologies with FhuA and other TonB-dependent receptors a topological model of FoxA is presented.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Outer Membrane Proteins / genetics*
  • Bacterial Outer Membrane Proteins / metabolism
  • Bacterial Proteins / metabolism
  • Base Sequence
  • Consensus Sequence
  • Deferoxamine / metabolism*
  • Escherichia coli / genetics
  • Escherichia coli Proteins*
  • Ferric Compounds / metabolism*
  • Genes, Bacterial*
  • Membrane Proteins / metabolism
  • Models, Molecular
  • Molecular Sequence Data
  • Multigene Family
  • Protein Conformation
  • Protein Sorting Signals / genetics
  • Receptors, Cell Surface*
  • Receptors, Drug / genetics*
  • Receptors, Drug / metabolism
  • Receptors, Virus / genetics
  • Recombinant Proteins / metabolism
  • Rhodobacter capsulatus / genetics
  • Sequence Homology, Nucleic Acid
  • Species Specificity
  • Yersinia enterocolitica / genetics
  • Yersinia enterocolitica / metabolism*

Substances

  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • Escherichia coli Proteins
  • Ferric Compounds
  • FhuA protein, E coli
  • FoxA protein, bacteria
  • FoxB protein, E coli
  • Membrane Proteins
  • Protein Sorting Signals
  • Receptors, Cell Surface
  • Receptors, Drug
  • Receptors, Virus
  • Recombinant Proteins
  • tonB protein, Bacteria
  • tonB protein, E coli
  • ferrioxamine B
  • Deferoxamine

Associated data

  • GENBANK/X60447