The Aeromonas Hydrophila exeE Gene, Required Both for Protein Secretion and Normal Outer Membrane Biogenesis, Is a Member of a General Secretion Pathway

Mol Microbiol. 1992 May;6(10):1351-61. doi: 10.1111/j.1365-2958.1992.tb00856.x.


The Aeromonas hydrophila Tn5-751 insertion mutant L1.97 is unable to secrete extracellular proteins, and is fragile because of defective assembly of its outer membrane. A KpnI 4.1 kb fragment, which complements this mutant when supplied with an exogenous promoter, was isolated and sequenced. It contains two complete genes, exeE and exeF, plus fragments of two others and may form part of an operon. The exeE and exeF open reading frames encode 501-residue M(r) 55,882 and 388-residue M(r) 43,431 proteins, respectively. These genes were expressed in vitro and their initiation codons verified by deletion analysis. Tn5-751 had inserted near the centre of the exeE gene in the L1.97 strain. Subclones of the KpnI 4.1 kb fragment which contained only the exeE gene fully complemented the mutation, indicating that its function is required both for extracellular secretion and outer membrane assembly. ExeE and ExeF are highly similar to other proteins which have been shown to be involved in extracellular secretion, suggesting that an additional export apparatus beyond that required for inner membrane translocation may be part of the physiology of many Gram-negative bacteria.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aeromonas hydrophila / genetics*
  • Aeromonas hydrophila / physiology
  • Amino Acid Sequence
  • Bacterial Outer Membrane Proteins / metabolism*
  • Bacterial Proteins*
  • Base Sequence
  • Cell Membrane / metabolism*
  • DNA, Bacterial / genetics
  • Genes, Bacterial*
  • Gram-Negative Bacteria / genetics
  • Gram-Negative Bacteria / metabolism
  • Klebsiella / genetics
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Open Reading Frames
  • Promoter Regions, Genetic
  • Recombinant Fusion Proteins / metabolism
  • Sequence Homology, Nucleic Acid


  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • DNA, Bacterial
  • Membrane Proteins
  • Recombinant Fusion Proteins
  • exeF protein, Aeromonas hydrophila
  • exeE protein, Aeromonas hydrophila