Characterisation of potentially probiotic vaginal lactobacilli isolated from Argentinean women

Br J Biomed Sci. 2005;62(4):170-4. doi: 10.1080/09674845.2005.11732706.


This study aims to evaluate potentially beneficial properties of 20 strains of vaginal lactobacilli isolated from women in Tucumán, Argentina, by determining acid and hydrogen peroxide production and auto-aggregation ability. The microorganisms were characterised genetically by amplified ribosomal 16S-DNA restriction analysis (ARDRA). Lactobacillus gasseri and L. rhamnosus were the predominant species identified among the 20 vaginal lactobacilli strains. Most achieved low pH values after 12 h incubation at 37 degrees C and produced hydrogen peroxide in static culture. However, pH decrease and semi-quantitative hydrogen peroxide production of most homofermentative lactobacilli were significantly higher than those of heterofermentative lactobacilli. Of the 20 strains studied, only three demonstrated remarkable auto-aggregation patterns. Four strains were selected for possible use in a probiotic product for vaginal application; however, further in vitro study of other potentially probiotic characteristics is required before attempting clinical trials.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Bacterial / genetics
  • Female
  • Humans
  • Hydrogen Peroxide / metabolism
  • Hydrogen-Ion Concentration
  • Lactobacillus / classification
  • Lactobacillus / isolation & purification*
  • Lactobacillus / metabolism
  • Probiotics*
  • RNA, Ribosomal, 16S / genetics
  • Vagina / microbiology*


  • DNA, Bacterial
  • RNA, Ribosomal, 16S
  • Hydrogen Peroxide