The Sm proteins regulate germ cell specification during early C. elegans embryogenesis

Dev Biol. 2006 Mar 1;291(1):132-43. doi: 10.1016/j.ydbio.2005.12.011. Epub 2006 Jan 18.

Abstract

Sm and Sm-like proteins are core components of the splicesome but have other functions distinct from pre-mRNA processing. Here, we show that Sm proteins also regulate germ cell specification during early C. elegans embryogenesis. SmE and SmG were required to maintain transcriptional quiescence in embryonic germ cell precursors. In addition, depletion of SmE inhibited expression of the germ lineage-specific proteins PIE-1, GLD-1, and NOS-2, but did not affect maintenance of several maternal mRNAs. PIE-1 had previously been shown to activate transcriptional silencing and NOS-2 expression. We found that PIE-1 also promotes GLD-1 expression by a process that is independent of transcriptional silencing. Thus, Sm proteins could control transcriptional silencing and maternal protein expression by regulating PIE-1. However, loss of SmE function also caused defects in P granule localization and premature division in early germline blastomeres, processes that are independent of PIE-1 function. Therefore, the Sm proteins control multiple aspects of germ cell precursor development. Because depletion of several other core splicing factors did not affect these events, these Sm functions are likely distinct from pre-mRNA splicing. Sm family proteins assemble into ribonucleoprotein complexes (RNPs) that control RNA activities. We suggest that novel Sm RNPs directly or indirectly influence posttranscriptional control of maternal mRNAs to promote germ cell specification in the early C. elegans embryo.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Caenorhabditis elegans / cytology
  • Caenorhabditis elegans / embryology*
  • Caenorhabditis elegans / metabolism
  • Caenorhabditis elegans Proteins / biosynthesis
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / physiology*
  • Embryo, Nonmammalian / cytology
  • Embryo, Nonmammalian / metabolism
  • Embryonic Development*
  • Gene Expression Regulation, Developmental
  • Germ Cells / cytology*
  • Germ Cells / metabolism
  • Nuclear Proteins / physiology
  • RNA Interference
  • RNA Splicing
  • RNA, Messenger / biosynthesis
  • RNA, Messenger, Stored / biosynthesis
  • Ribonucleoproteins, Small Nuclear / genetics
  • Ribonucleoproteins, Small Nuclear / physiology*

Substances

  • Caenorhabditis elegans Proteins
  • GLD-1 protein, C elegans
  • Nuclear Proteins
  • RNA, Messenger
  • RNA, Messenger, Stored
  • Ribonucleoproteins, Small Nuclear
  • nos-2 protein, C elegans
  • pie-1 protein, C elegans