Enhanced immunogenicity of microencapsulated multiepitope DNA vaccine encoding T and B cell epitopes of foot-and-mouth disease virus in mice

Vaccine. 2006 Mar 15;24(12):2017-26. doi: 10.1016/j.vaccine.2005.11.042. Epub 2005 Dec 1.

Abstract

The role of poly(D,L-lactic-co-glycolic acid, PLGA) microparticles on enhancing immune responses of multiepitope DNA vaccines was investigated in vitro and in vivo. pcDNA-SG encoding T and B cell epitopes of foot-and-mouth disease virus (FMDV) was encapsulated into PLGA microparticles. PLGA microparticles could protect themselves from nuclease degradation in vitro. PLGA-pcDNA-SG microparticles could be uptaken by cells and expressed His-tagged SG immunogen in vitro and in vivo. A prolonged expression and presentation of SG immunogen were observed by confocal laser scanning microscopy in the lymphocytes from the mice incubated with PLGA-pcDNA-SG microparticles, compared with the mice immunized with naked pcDNA-SG. PLGA-pcDNA-SG microparticles displayed a significant stronger immunogenicity than naked DNA vaccines with a higher titer of virus-specific antibody, elevated IFN-gamma production and enhanced lymphocyte proliferation. PLGA-DNA microparticle could elicit augmented humoral and cellular responses with reduced amounts and times of immunization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Drug Compounding
  • Drug Delivery Systems
  • Epitopes, B-Lymphocyte / genetics*
  • Epitopes, T-Lymphocyte / genetics*
  • Foot-and-Mouth Disease / immunology
  • Foot-and-Mouth Disease / prevention & control*
  • Foot-and-Mouth Disease / virology
  • Foot-and-Mouth Disease Virus / genetics*
  • Foot-and-Mouth Disease Virus / immunology
  • Immunity, Cellular / drug effects*
  • Mice
  • Nanostructures
  • Vaccination / methods
  • Vaccines, DNA / administration & dosage*
  • Vaccines, DNA / genetics
  • Viral Vaccines / administration & dosage
  • Viral Vaccines / immunology

Substances

  • Epitopes, B-Lymphocyte
  • Epitopes, T-Lymphocyte
  • Vaccines, DNA
  • Viral Vaccines