[Molecular biological identification of Batai virus isolated in China]

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2005 Dec;19(4):331-4.
[Article in Chinese]


Background: To study the molecular characteristics of YN92-4 strain isolated from mosquitoes in Yunnan Province and define its classification.

Methods: The S segment of YN92-4 strain was amplified and sequenced by 2 different sets of primers. The phylogenic tree of S fragment was constructed by Phylip bio-software. The amino acid sequences of N and NSs proteins were also studied.

Results: YN92-4 strain could be amplified by 2 sets of primers respectively, S segment showed a highest homology with Batai virus (X73464), reached 96.4%, the homology of protein N and NSs amio-acid sequence with Batai virus was 99.1% and 98% respectively.

Conclusion: The YN92-4 strain belongs to Batai virus, this is the first report of molecular biological identification of Batai virus in China.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Bunyamwera virus / classification
  • Bunyamwera virus / genetics*
  • Bunyamwera virus / isolation & purification*
  • China
  • Culicidae / virology*
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Viral / genetics
  • RNA, Viral / isolation & purification
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid


  • DNA, Complementary
  • RNA, Viral