Induction of pathogenic anti-dsDNA antibodies is controlled on the level of B cells in a non-lupus prone mouse strain

J Clin Immunol. 2006 Jan;26(1):86-95. doi: 10.1007/s10875-006-8904-y.


The SmD1(83-119) peptide is a main target of autoantibodies and T cells in human and murine lupus, but its role in autoimmunity induction remains elusive. Therefore, female Balb/c mice and (NZW x Balb/c)F1 [CWF1] mice with identical MHC haplotype as lupus prone NZB/W mice were immunized with SmD1(83-119). Immunizations of CWF1 mice with SmD1(83-119), but not with the controls (irrelevant peptide, HEL peptide, or saline), induced anti-SmD1(83-119) and anti-dsDNA antibodies and proteinuria not present in Balb/c mice. DsDNA-specific plasma cell induction after SmD1(83-119) immunizations was confirmed by ELISPOT assays showing that the generation of dsDNA-specific antibody forming cells (AFC) was mainly driven by increased T-cell help. T-cell help for the generation of dsDNA-specific AFC was also present in saline-treated CWF1 mice but was controlled on the levels of B cells preventing autoimmunity.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Antibodies, Antinuclear / biosynthesis*
  • B-Lymphocytes / immunology*
  • DNA / immunology*
  • Female
  • Immunization
  • Lupus Vulgaris / genetics*
  • Lupus Vulgaris / immunology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred NZB
  • Peptide Fragments / immunology
  • Ribonucleoproteins, Small Nuclear / immunology*
  • T-Lymphocytes / immunology
  • snRNP Core Proteins


  • Antibodies, Antinuclear
  • Peptide Fragments
  • Ribonucleoproteins, Small Nuclear
  • Snrpd1 protein, mouse
  • snRNP Core Proteins
  • DNA