Structures of the core oligosaccharide and O-units in the R- and SR-type lipopolysaccharides of reference strains of Pseudomonas aeruginosa O-serogroups

FEMS Immunol Med Microbiol. 2006 Feb;46(1):85-99. doi: 10.1111/j.1574-695X.2005.00004.x.


Highly phosphorylated core oligosaccharides and those substituted with one O-antigen repeating unit were obtained by mild acid degradation or strong alkaline hydrolysis of lipopolysaccharide samples from 23 reference strains representing all Pseudomonas aeruginosa O-serogroups. Studies by high-resolution electrospray ionization mass spectrometry and two-dimensional NMR spectroscopy revealed both conserved and variable structural features of the lipopolysaccharides of various O-serogroups. The upstream terminal saccharide of the O-antigen, which contributes most to the immunospecificity of the bacteria, was defined in 11 from a total of 13 O-serogroups. The data obtained link together the known biosynthesis pathways, genetics and serology of the P. aeruginosa lipopolysaccharide.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Lipopolysaccharides / chemistry*
  • Magnetic Resonance Spectroscopy
  • Molecular Sequence Data
  • O Antigens / chemistry*
  • Oligosaccharides / chemistry*
  • Pseudomonas aeruginosa / immunology*
  • Spectrometry, Mass, Electrospray Ionization


  • Lipopolysaccharides
  • O Antigens
  • Oligosaccharides