The reductive dechlorination of 2,3,4,5-tetrachlorobiphenyl in three different sediment cultures: evidence for the involvement of phylogenetically similar Dehalococcoides-like bacterial populations

FEMS Microbiol Ecol. 2006 Feb;55(2):248-61. doi: 10.1111/j.1574-6941.2005.00022.x.

Abstract

Anaerobic cultures capable of reductively dechlorinating 2,3,4,5-tetrachlorobiphenyl (CB) were enriched from three different sediments, one estuarine, one marine and one riverine. Two different electron donors were used in enrichments with the estuarine sediment (elemental iron or a mixture of fatty acids). The removal of doubly flanked meta and para chlorines to form 2,3,5-CB and 2,4,5-CB was observed in all cultures. Bacterial community analysis of PCR-amplified 16S rRNA gene fragments revealed different communities in these cultures, with the exception of one common population that showed a high phylogentic relatedness to Dehalococcoides species. No Dehalococcoides-like populations were ever detected in control cultures to which no PCBs were added. In addition, the dynamics of this Dehalococcoides-like population were strongly correlated with dechlorination. Subcultures of the estuarine sediment culture demonstrated that the Dehalococcoides-like population disappeared when dechlorination was inhibited with 2-bromoethanesulfonate or when 2,3,4,5-CB had been consumed. These results provide evidence that Dehalococcoides-like populations were involved in the removal of doubly flanked chlorines from 2,3,4,5-CB. Furthermore, the successful enrichment of these populations from geographically distant and geochemically distinct environments indicates the widespread presence of these PCB-dechlorinating, Dehalococcoides-like organisms.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Alkanesulfonic Acids / pharmacology
  • Baltimore
  • Biodiversity
  • Chloroflexi / classification
  • Chloroflexi / genetics
  • Chloroflexi / isolation & purification*
  • Chloroflexi / metabolism*
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Electrophoresis, Polyacrylamide Gel
  • Fatty Acids / metabolism
  • Geologic Sediments / microbiology*
  • Iron / metabolism
  • Molecular Sequence Data
  • Oxidation-Reduction
  • Phylogeny
  • Polychlorinated Biphenyls / metabolism*
  • Polymerase Chain Reaction
  • RNA, Ribosomal, 16S / genetics
  • Seawater / microbiology
  • Sequence Analysis, DNA
  • Water Microbiology*

Substances

  • Alkanesulfonic Acids
  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • RNA, Ribosomal, 16S
  • 2-bromoethanesulfonic acid
  • 2,3,4,5-tetrachlorobiphenyl
  • Polychlorinated Biphenyls
  • Iron

Associated data

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