Anti-viral protein APOBEC3G is induced by interferon-alpha stimulation in human hepatocytes

Biochem Biophys Res Commun. 2006 Mar 10;341(2):314-9. doi: 10.1016/j.bbrc.2005.12.192. Epub 2006 Jan 10.


Apolipoprotein B mRNA-editing enzyme catalytic-polypeptide 3G (APOBEC3G) is a potent inhibitor of infection by a wide range of retroviruses. Although recent reports have suggested that human APOBEC3G exerts antiviral activity against hepatitis B virus, APOBEC3G expression is normally low in the human liver. To clarify the role of APOBEC3G in cellular defenses against hepatitis viruses, the regulation of the APOBEC3G expression was investigated in human hepatocytes. Endogenous transcripts of nine APOBEC family members were barely detectable in quiescent liver cells. However, APOBEC3G was significantly up-regulated in response to interferon-alpha (IFN-alpha) stimulation in HepG2, Huh-7, and primary human hepatocytes. IFN regulatory factor elements that are important for IFN-inducible promoter activity were identified 5' upstream from the human APOBEC3G gene. Our findings provided the first evidence showing that APOBEC3G is induced by IFN stimulation in human hepatocytes and thus could be involved in host defense mechanisms directed against hepatitis viruses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • APOBEC-3G Deaminase
  • Antiviral Agents / chemistry*
  • Base Sequence
  • Carcinoma, Hepatocellular / metabolism
  • Cell Line
  • Cytidine Deaminase
  • DNA Primers / chemistry
  • Gene Expression Regulation
  • Hepatitis Viruses / metabolism
  • Hepatocytes / metabolism
  • Humans
  • Immunoblotting
  • Interferon-alpha / metabolism*
  • Luciferases / metabolism
  • Models, Genetic
  • Molecular Sequence Data
  • Nucleoside Deaminases / metabolism
  • Nucleoside Deaminases / physiology*
  • Plasmids / metabolism
  • Promoter Regions, Genetic
  • RNA / metabolism
  • Repressor Proteins / metabolism
  • Repressor Proteins / physiology*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Time Factors


  • Antiviral Agents
  • DNA Primers
  • Interferon-alpha
  • Repressor Proteins
  • RNA
  • Luciferases
  • Nucleoside Deaminases
  • APOBEC-3G Deaminase
  • APOBEC3G protein, human
  • Cytidine Deaminase