Identification of sn-glycerol-1-phosphate dehydrogenase activity from genomic information on a hyperthermophilic archaeon, Sulfolobus tokodaii strain 7

Biosci Biotechnol Biochem. 2006 Jan;70(1):282-5. doi: 10.1271/bbb.70.282.

Abstract

sn-Glycerol-1-phosphate dehydrogenase is responsible for the formation of sn-glycerol-1-phosphate, the backbone of membrane phospholipids of Archaea. This activity had never been detected in cell-free extract of Sulfolobus sp. Here we report the detection of this activity on the thermostable ST0344 protein of Sulfolobus tokodaii expressed in Escherichia coli, which was predicted from genomic information on S. tokodaii. This is another line of evidence for the general mechanism of sn-glycerol-1-phosphate formation by the enzyme.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Conserved Sequence
  • Enzyme Stability
  • Gene Expression
  • Genome, Archaeal / genetics*
  • Glycerolphosphate Dehydrogenase / chemistry
  • Glycerolphosphate Dehydrogenase / genetics
  • Glycerolphosphate Dehydrogenase / isolation & purification
  • Glycerolphosphate Dehydrogenase / metabolism*
  • Glycerophosphates / metabolism
  • Hydrogen-Ion Concentration
  • Molecular Sequence Data
  • NADP / metabolism
  • Oxidation-Reduction
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Substrate Specificity
  • Sulfolobus / classification
  • Sulfolobus / enzymology*
  • Sulfolobus / genetics*
  • Temperature

Substances

  • Glycerophosphates
  • Recombinant Proteins
  • NADP
  • Glycerolphosphate Dehydrogenase
  • glycerol-1-phosphate dehydrogenase