Immunoreactive ACTH and cortisol plasma levels during pregnancy. Detection and partial purification of corticotrophin-like placental hormone: the human chorionic corticotrophin (HCC)

Clin Endocrinol (Oxf). 1975 Jan;4(1):1-14. doi: 10.1111/j.1365-2265.1975.tb03299.x.


The high plasma cortisol and ACTH levels present in pregnant women as well as the non-parrallelism of their plasma extract dilution curves in comparison with the standard curve in the ACTH radioimmunoassay, are evidence for the presence of an ACTH-like substance during pregnancy which would interfere with the assay. Placental extracts were obtained by acid-acetone extraction, followed by partial purification with oxycellulose and by extraction with porous glass powder. A substance was detected which partially cross-reacted with synthetic human ACTH in the radioimmunoassay and which showed biological activity using the assay procedure described by Liscomb & Nelson. The data sustain the existence of an ACTH-like placental hormone: human chorionic corticotrophin (HCC).

MeSH terms

  • Adrenocorticotropic Hormone / blood*
  • Adrenocorticotropic Hormone / immunology
  • Adrenocorticotropic Hormone / isolation & purification*
  • Animals
  • Biological Assay
  • Corticosterone / analysis
  • Cross Reactions
  • Culture Techniques
  • Female
  • Humans
  • Hydrocortisone / blood*
  • Placental Hormones / isolation & purification*
  • Pregnancy*
  • Rabbits / immunology
  • Radioimmunoassay
  • Rats


  • Placental Hormones
  • Adrenocorticotropic Hormone
  • Corticosterone
  • Hydrocortisone