Plasma membrane of Synechocystis PCC 6803: a heterogeneous distribution of membrane proteins

Arch Microbiol. 2006 Apr;185(3):238-43. doi: 10.1007/s00203-006-0086-8. Epub 2006 Jan 24.


Proteomic studies carried out previously on the plasma membrane of Synechocystis have identified several peripheral and integral proteins. The distribution of these proteins along the membrane still remains obscure. In this study, the distribution of proteins along the plasma membrane of Synechocystis was carried out using subfractions, the right-side-out (RSO) and inside-out (ISO) vesicles, fractionated from a pure and specific fraction of the plasma membrane. These subfractions were analyzed and quantified for several proteins by immunoblotting. It was found that the ISO fraction contained higher quantities of preD1, D1 and PsaD, the integral proteins of photosystem I and II known to be present also in the plasma membrane. Lower amounts of peripheral vesicle inducing protein Vipp1 and nitrate/nitrite binding protein NrtA were present in the ISO compared to the RSO fraction. On the contrary, the distribution of two integral transporter proteins, SbtA and PxcA, was found equal in both fractions. Our studies clearly establish that the plasma membrane of Synechocystis has a heterogeneous composition with respect to protein distribution. The accumulation of photosynthesis-associated proteins in the ISO fraction provides evidence that the discrete regions of the plasma membrane harbor sites for biogenesis of photosystems.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Outer Membrane Proteins / chemistry
  • Bacterial Outer Membrane Proteins / metabolism
  • Bacterial Proteins / analysis*
  • Bacterial Proteins / metabolism
  • Cell Membrane / chemistry*
  • Cell Membrane / metabolism
  • Membrane Proteins / analysis*
  • Membrane Proteins / metabolism
  • Photosynthesis
  • Subcellular Fractions
  • Synechocystis / chemistry
  • Synechocystis / metabolism*
  • Thylakoids / chemistry
  • Thylakoids / metabolism
  • Trypsin / metabolism


  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • Membrane Proteins
  • Trypsin