Discontinuous epitope prediction based on mimotope analysis

Bioinformatics. 2006 May 1;22(9):1088-95. doi: 10.1093/bioinformatics/btl012. Epub 2006 Jan 24.


Motivation: Phage display is a widespread technique used to obtain peptide mimotopes selected by binding to a given monoclonal antibody in a similar way as the native epitope. However, the localization of the interaction site mimicked by the mimotopes on the surface of the antigen is not always a straightforward task. MIMOP is a computational tool developed with the aim of helping experimentalists to analyze a set of mimotope sequences and guide them in the identification of the mimicked region.

Results: To predict potential epitopic regions, MIMOP integrates two different approaches combining two- and three-dimensional analyses: MimAlign starts from degenerated alignment analyses, and MimCons is based on consensus identification. The relevance and usefulness of the tool are illustrated by four use cases corresponding to real-life situations.

MeSH terms

  • Algorithms*
  • Amino Acid Sequence
  • Binding Sites
  • Epitope Mapping / methods*
  • Epitopes / chemistry*
  • Epitopes / immunology*
  • Molecular Sequence Data
  • Protein Binding
  • Sequence Alignment / methods*
  • Sequence Analysis, Protein / methods*


  • Epitopes