Multilocus sequence typing (MLST) analysis of Vibrio cholerae O1 El Tor isolates from Mozambique that harbour the classical CTX prophage

J Med Microbiol. 2006 Feb;55(Pt 2):165-170. doi: 10.1099/jmm.0.46287-0.


Vibrio cholerae O1 isolates belonging to the Ogawa serotype, El Tor biotype, harbouring the classical CTX prophage were first isolated in Mozambique in 2004. Multilocus sequence typing (MLST) analysis using nine genetic loci showed that the Mozambique isolates have the same sequence type (ST) as O1 El Tor N16961, a representative of the current seventh cholera pandemic. Analysis of the CTX prophage in the Mozambique isolates indicated that there is one type of rstR in these isolates: the classical CTX prophage. It was also found that the ctxB-rstR-rstA-rstB-phs-cep fragment was PCR-amplified from these isolates, which indicates the presence of a tandem repeat of the classical CTX prophage in the genome of the Mozambique isolates. The possible origin of these isolates and the presence of the tandem repeat of the classical prophage in them implicate the presence of the classical CTX phage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics
  • Bacteriophages / genetics
  • Genetic Variation
  • Genome, Bacterial / genetics
  • Humans
  • Molecular Sequence Data
  • Mozambique
  • Prophages / genetics
  • Repressor Proteins / genetics
  • Sequence Analysis, DNA
  • Species Specificity
  • Tandem Repeat Sequences / genetics
  • Vibrio cholerae O1 / classification*
  • Vibrio cholerae O1 / genetics


  • Bacterial Proteins
  • Repressor Proteins
  • RstR protein, Vibrio cholerae