Pneumococcal surface protein A (PspA) family distribution among clinical isolates from adults over 50 years of age collected in seven countries

J Med Microbiol. 2006 Feb;55(Pt 2):215-221. doi: 10.1099/jmm.0.46268-0.


The pneumococcal surface protein PspA, a cell-wall-associated surface protein, is a promising component for pneumococcal vaccines. In this study, the distribution of the PspA family was determined in a panel of invasive and clinically important pneumococcal isolates from adults over 50 years of age, collected between 1995 and 2002. One thousand eight hundred and forty-seven recent isolates from invasive pneumococcal disease were obtained from seven Western countries, together with clinical data. An ELISA-based serological method was standardized in order to determine the PspA family and clade distribution. Molecular tests were used when isolates were non-typable by ELISA (PspA family typing by PCR). Only 42 (2.3 %) isolates were non-typable by ELISA and PspA family typing by PCR was performed. Finally, 3 isolates were considered as non-pneumococcal and 1844 were classified as follows: 749 (40.6 %) were PspA family 1, 1078 (58.5 %) were PspA family 2, 13 (0.7 %) were PspA family 1 and 2 and 4 (0.2 %) remained non-typable. The cross-reactivity of antibodies to PspAs of different clades was confirmed. In conclusion, inclusion of PspA family 1 and family 2 in future pneumococcal vaccines would ensure broad coverage of pneumococcal strains infecting people over 50 years of age.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Antibodies, Bacterial / immunology
  • Australia
  • Bacterial Proteins / classification*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / immunology
  • Canada
  • Cross Reactions
  • Enzyme-Linked Immunosorbent Assay
  • Europe
  • Female
  • Humans
  • Male
  • Middle Aged
  • Pneumococcal Infections / microbiology*
  • Polymerase Chain Reaction
  • Streptococcus pneumoniae / chemistry*
  • Streptococcus pneumoniae / immunology
  • United States


  • Antibodies, Bacterial
  • Bacterial Proteins
  • pneumococcal surface protein A