Cell refractive index tomography by digital holographic microscopy

Opt Lett. 2006 Jan 15;31(2):178-80. doi: 10.1364/ol.31.000178.


For what we believe to be the first time, digital holographic microscopy is applied to perform optical diffraction tomography of a pollen grain. Transmission phase images with nanometric axial accuracy are numerically reconstructed from holograms acquired for different orientations of the rotating sample; then the three-dimensional refractive index spatial distribution is computed by inverse radon transform. A precision of 0.01 for the refractive index estimation and a spatial resolution in the micrometer range are demonstrated.

Publication types

  • Evaluation Study

MeSH terms

  • Algorithms
  • Holography / methods*
  • Image Enhancement / methods
  • Image Interpretation, Computer-Assisted / methods
  • Imaging, Three-Dimensional / methods*
  • Microscopy / methods*
  • Pollen / ultrastructure*
  • Refractometry / methods*
  • Signal Processing, Computer-Assisted*
  • Tomography, Optical / methods*