Read-through transcript from NM23-H1 into the neighboring NM23-H2 gene encodes a novel protein, NM23-LV

Genomics. 2006 Apr;87(4):483-9. doi: 10.1016/j.ygeno.2005.11.004. Epub 2006 Jan 25.


NM23-H1 and NM23-H2 are neighboring genes on chromosome 17q. They encode nucleoside diphosphate kinases that have additional roles in signal transduction, transcription, and apoptosis. NM23-H1 expression is a strong marker for prognosis and metastatic behavior in many tumor types. A new bioinformatic tool, TranscriptView, identified read-through transcripts that start in the NM23-H1 gene and continue in the neighboring NM23-H2 gene. Splicing results in a transcript containing exons 1 to 4 of NM23-H1 and exons 2 to 5 of NM23-H2. The resulting mRNA encodes a novel and long variant of the NM23 protein family, NM23-LV, which contains part of NM23-H1 and the complete NM23-H2 protein. The transcript was amplified and sequenced from two neuroblastoma cell lines, confirming the presence of the predicted NM23-LV mRNA in vivo. Tissue analysis showed that NM23-LV is ubiquitously expressed, with the exception of the kidney. Neuroblastoma tumors show high-level expression of NM23-H1 and-H2 as well as NM23-LV mRNA. In neuroblastoma cells, the NM23-LV protein has mainly a cytoplasmic localization, but some nuclear staining was observed as well.

MeSH terms

  • Alternative Splicing*
  • Amino Acid Sequence
  • Biomarkers, Tumor
  • Blotting, Western
  • Cell Line, Tumor
  • Cell Nucleus / metabolism
  • Chromosomes, Human, Pair 17
  • Computational Biology
  • Cytoplasm / metabolism
  • DNA, Neoplasm / genetics
  • Exons
  • Expressed Sequence Tags
  • Fluorescent Antibody Technique
  • Fluorescent Dyes
  • Gene Expression Regulation, Neoplastic*
  • Genetic Variation
  • Humans
  • Introns
  • Molecular Sequence Data
  • NM23 Nucleoside Diphosphate Kinases
  • Neuroblastoma / genetics
  • Nucleic Acid Amplification Techniques
  • Nucleoside-Diphosphate Kinase / genetics*
  • RNA, Messenger / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rhodamines
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Transcription, Genetic*


  • Biomarkers, Tumor
  • DNA, Neoplasm
  • Fluorescent Dyes
  • NM23 Nucleoside Diphosphate Kinases
  • RNA, Messenger
  • Rhodamines
  • tetramethylrhodamine isothiocyanate
  • NME1 protein, human
  • Nucleoside-Diphosphate Kinase

Associated data

  • GENBANK/DQ109675