Plasmids with easily excisable xylE cassettes

Gene. 1992 Aug 1;117(1):157-8. doi: 10.1016/0378-1119(92)90506-k.

Abstract

Two new vectors containing the xylE gene (encoding catechol-2,3-dioxygenase) of Pseudomonas putida were constructed that serve as the source of the xylE cassette. These vectors are based on the kanamycin-resistance-encoding plasmid, pKAN18. The promoter-less xylE gene is flanked by several restriction enzyme sites that allow for easy excision of this gene in the form of a cassette containing a ribosome-binding site, 7 bp upstream from the start codon. These cassettes lack any transcriptional termination signals downstream from the stop codon.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Catechol 2,3-Dioxygenase
  • Cloning, Molecular
  • DNA, Bacterial
  • Dioxygenases*
  • Molecular Sequence Data
  • Oxygenases / genetics*
  • Plasmids / genetics*
  • Pseudomonas putida / enzymology
  • Pseudomonas putida / genetics*
  • Restriction Mapping

Substances

  • DNA, Bacterial
  • Oxygenases
  • Dioxygenases
  • Catechol 2,3-Dioxygenase