Sequence of a staphylococcal plasmid gene, vga, encoding a putative ATP-binding protein involved in resistance to virginiamycin A-like antibiotics

Gene. 1992 Aug 1;117(1):45-51. doi: 10.1016/0378-1119(92)90488-b.

Abstract

The Staphylococcus aureus plasmid gene, vga, conferring resistance to A compounds of virginiamycin-like antibiotics (streptogramin A, pristinamycin II, virginiamycin M), and to synergistic mixtures of the A and B compounds of these antibiotics, was cloned and sequenced. This gene potentially encodes a 522-amino acid protein, VgA, of 60,115 Da which exhibits significant homology with the ATP-binding domains of numerous proteins. VgA has two ATP-binding domains, containing each the A and the B motifs, but does not contain long hydrophobic stretches that might represent potential membrane-spanning domains. Resistance to A compounds of virginiamycin-like antibiotics conferred to S. aureus by gene vga was not conferred to Escherichia coli, although a protein having an M(r) similar to that encoded by this gene was detected in E. coli minicells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters*
  • Adenosine Triphosphate / metabolism*
  • Amino Acid Sequence
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Base Sequence
  • Cloning, Molecular
  • DNA, Bacterial
  • Drug Resistance, Microbial / genetics
  • Molecular Sequence Data
  • Plasmids / genetics*
  • Protein Binding
  • Restriction Mapping
  • Staphylococcus aureus / genetics*
  • Virginiamycin / pharmacology*

Substances

  • ATP-Binding Cassette Transporters
  • Bacterial Proteins
  • DNA, Bacterial
  • Virginiamycin
  • VgA protein, Staphylococcus aureus
  • Adenosine Triphosphate

Associated data

  • GENBANK/M73501
  • GENBANK/M73502
  • GENBANK/M73503
  • GENBANK/M73504
  • GENBANK/M74891
  • GENBANK/M74892
  • GENBANK/M79309
  • GENBANK/M79310
  • GENBANK/M90056
  • GENBANK/M90357