Intra-adrenal interactions in fish: catecholamine stimulated cortisol release in sea bass (Dicentrarchus labrax L.)

Comp Biochem Physiol A Mol Integr Physiol. 2006 Mar;143(3):375-81. doi: 10.1016/j.cbpa.2005.12.027. Epub 2006 Jan 27.


The effect of the catecholamines, adrenaline and noradrenaline, on sea bass (Dicentrarchus labrax) and sea bream (Sparus auratus) interrenal cortisol production was studied in vitro using a dynamic superfusion system technique. Increasing concentrations of catecholamines (10(-6), 10(-8) and 10(-10) M) stimulated cortisol production in a dose-dependent manner, in sea bass only. The increase in cortisol production stimulated by adrenaline (10(-6) M) and noradrenaline (10(-6) M) was inhibited by sotalol (2 x 10(-5) M), but not by prazosin suggesting that catecholamines stimulate cortisol release through the beta-receptor subtype. To evaluate catecholamine-induced signal transduction in head kidney cells, measurements of cAMP production and [H3]myo-inositol incorporation were determined in head kidney cell suspensions. Adrenaline and noradrenaline (10(-6) M) increased cAMP production, but had no effect on total inositol phosphate accumulation. These results indicate that catecholamines released from the chromaffin cells within the interrenal tissue may act as a paracrine factor to stimulate interrenal steroidogenesis in the sea bass.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenergic alpha-Antagonists / pharmacology
  • Adrenergic beta-Antagonists / pharmacology
  • Animals
  • Bass / physiology*
  • Epinephrine / pharmacology*
  • Hydrocortisone / metabolism*
  • Inositol / metabolism
  • Interrenal Gland / physiology*
  • Norepinephrine / pharmacology*
  • Prazosin / pharmacology
  • Second Messenger Systems / physiology
  • Sotalol / pharmacology


  • Adrenergic alpha-Antagonists
  • Adrenergic beta-Antagonists
  • Inositol
  • Sotalol
  • Hydrocortisone
  • Norepinephrine
  • Prazosin
  • Epinephrine