Acute liver damage induced by 2-nitropropane in rats: effect of diphenyl diselenide on antioxidant defenses

Chem Biol Interact. 2006 Mar 25;160(2):99-107. doi: 10.1016/j.cbi.2005.12.010. Epub 2006 Jan 30.


The effect of post-treatment with diphenyl diselenide on liver damage induced by 2-nitropropane (2-NP) was examined in male rats. Rats were pre-treated with a single dose of 2-NP (100 mg/kg body weight dissolved in canola oil). Afterward, the animals were post-treated with a dose of diphenyl diselenide (10, 50 or 100 micromol/kg). The parameters that indicate tissue damage such as liver histopathology, plasma aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transferase (GGT), urea and creatinine were determined. Since the liver damage induced by 2-NP is related to oxidative damage, lipid peroxidation, superoxide dismutase (SOD), catalase (CAT) and ascorbic acid level were also evaluated. Diphenyl diselenide (50 and 100 micromol/kg) effectively restored the increase of ALT and AST activities and urea level when compared to the 2-NP group. At the higher dose, diphenyl diselenide decreased GGT activity. Treatment with diphenyl diselenide, at all doses, effectively ameliorated the increase of hepatic and renal lipid peroxidation when compared to 2-NP group. 2-NP reduced CAT activity and neither alter SOD activity nor ascorbic acid level. This study points out the involvement of CAT activity in 2-NP-induced acute liver damage and suggests that the post-treatment with diphenyl diselenide was effective in restoring the hepatic damage induced by 2-NP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Animals
  • Antioxidants / pharmacology*
  • Benzene Derivatives / pharmacology*
  • Chemical and Drug Induced Liver Injury / metabolism
  • Chemical and Drug Induced Liver Injury / pathology
  • Chemical and Drug Induced Liver Injury / prevention & control*
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Drug Interactions
  • Enzymes / metabolism
  • Free Radical Scavengers
  • Kidney / drug effects
  • Kidney / metabolism
  • Lipid Peroxidation / drug effects
  • Liver / drug effects*
  • Liver / metabolism
  • Liver / pathology
  • Liver Function Tests
  • Male
  • Nitroparaffins / toxicity*
  • Organoselenium Compounds / pharmacology*
  • Propane / analogs & derivatives*
  • Propane / toxicity
  • Rats
  • Rats, Wistar
  • Reactive Oxygen Species / metabolism
  • Solvents / toxicity*


  • Antioxidants
  • Benzene Derivatives
  • Enzymes
  • Free Radical Scavengers
  • Nitroparaffins
  • Organoselenium Compounds
  • Reactive Oxygen Species
  • Solvents
  • diphenyldiselenide
  • 2-nitropropane
  • Propane