A new specific gene expression in squamous cell carcinoma of the esophagus detected using representational difference analysis and cDNA microarray

Oncology. 2006;70(1):25-33. doi: 10.1159/000091183. Epub 2006 Jan 27.


Objectives: To detect new specific gene expressions in squamous cell carcinoma of the esophagus.

Methods: Representational difference analysis of cDNA (cDNA RDA) was applied to a human esophageal cancer cell line (KYSE170) and a human esophageal epithelial cell line (HEEC-1).

Results: LAGE-1 was expressed specifically in KYSE170, but not in HEEC-1. It is also expressed in 27% of esophageal cancer cell lines (3/11) and 33% of esophageal cancer tissues (10/30), but not in other HEECs, normal esophageal epithelium, or other normal tissues except testis, ovary and kidney. The expression of LAGE-1 is strongly correlated with that of MAGE-A1 (p = 0.013, Fisher's exact probability test). Fibronectin, cytokeratin 6B, cytokeratin 19, cyclin D2 and Ten-m2 were detected as candidates for downregulated genes. Reduced expression profiles of them were also identified using cDNA microarrays. The expression of LAGE-1 was induced by 5'-aza-2'-deoxycytidine (5Aza-dC) and trichostatin A (TSA) in esophageal cancer cell lines, which did not express LAGE-1. In HEECs, 5Aza-dC induced LAGE-1 expression, but TSA did not.

Conclusions: LAGE-1 expression was detected in esophageal cancer by cDNA RDA. LAGE-1 might have the potential to be a target antigen for anti-tumoral immunotherapy in esophageal cancers because of its tumor-specific expression similar to that of MAGE-A1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Neoplasm / analysis*
  • Antigens, Surface
  • Biomarkers, Tumor / analysis*
  • Carcinoma, Squamous Cell / chemistry
  • Carcinoma, Squamous Cell / genetics*
  • Cell Line, Tumor
  • Cells, Cultured
  • Epithelial Cells
  • Esophageal Neoplasms / chemistry
  • Esophageal Neoplasms / genetics*
  • Esophagus / chemistry
  • Esophagus / cytology
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Membrane Proteins / analysis*
  • Oligonucleotide Array Sequence Analysis*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA


  • Antigens, Neoplasm
  • Antigens, Surface
  • Biomarkers, Tumor
  • CTAG1B protein, human
  • CTAG2 protein, human
  • Membrane Proteins