Abstract
The gene nagC encodes the repressor for the nag regulon. A point mutation within the gene, which confers a super-repressor phenotype and makes the repressor insensitive to the inducer, N-acetylglucosamine 6-phosphate, has been characterized. The mutation is semi-dominant since heterozygous diploids have reduced growth rates on glucosamine and N-acetylglucosamine compared to the wild-type strain.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Acetylglucosamine / analogs & derivatives
-
Acetylglucosamine / metabolism
-
Amidohydrolases / biosynthesis*
-
Amidohydrolases / genetics
-
Amino Acyl-tRNA Synthetases / genetics
-
Bacterial Proteins / genetics*
-
Chromosome Mapping
-
Cloning, Molecular
-
Diploidy
-
Enzyme Induction / genetics
-
Escherichia coli / genetics*
-
Escherichia coli Proteins*
-
Gene Expression Regulation, Bacterial*
-
Genes, Dominant / genetics
-
Glucosamine / metabolism
-
Mutation / genetics
-
Recombinant Fusion Proteins / biosynthesis
-
Repressor Proteins / genetics*
-
Transcription Factors*
-
Transduction, Genetic
-
beta-Galactosidase / biosynthesis
Substances
-
Bacterial Proteins
-
Escherichia coli Proteins
-
Recombinant Fusion Proteins
-
Repressor Proteins
-
Transcription Factors
-
nagC protein, E coli
-
N-acetylglucosamine 6-phosphate
-
beta-Galactosidase
-
Amidohydrolases
-
N-acetylglucosamine-6-phosphate deacetylase
-
Amino Acyl-tRNA Synthetases
-
glutaminyl-tRNA synthetase
-
Glucosamine
-
Acetylglucosamine