Detection of virulence factors of Escherichia coli from children by multiplex polymerase chain reaction

Int J Clin Pract. 2006 Feb;60(2):170-3. doi: 10.1111/j.1742-1241.2005.00668.x.

Abstract

A total of 161 Escherichia coli (E. coli) strains isolated from children with urinary tract infection (UTI) were analysed for the genes encoding the virulence factors such as pyelonephritis (pap), s fimbriae (sfa), afimbrial adhesin I (afaI), haemolysin (hly), cytotoxic necrotising factor I (cnf I) and aerobactin (aer) by multiplex PCR. Ninety-four E. coli strains were found to carry at least one virulence factor. Therefore, 58.38% of total population was positive for one virulence gene at least. Percentage of genes within the total population for pap, sfa, afaI, hly, cnf I and aer was found as 22.98, 6.21, 9.94, 1.24, 9.94 and 39.75, respectively. Our analysis showed that sfa-pap (p < 0.001); pap-aer, afaI-aer and cnf I-pap (P < 0.05) and hly-sfa (p < 0.01) significantly co-occurred in their respective samples. In the light of these findings, we suggest an important role of pap causing UTI.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Child
  • DNA, Bacterial / analysis
  • Escherichia coli / genetics
  • Escherichia coli / isolation & purification
  • Escherichia coli / pathogenicity*
  • Escherichia coli Infections / microbiology*
  • Gene Frequency
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods
  • Urinary Tract Infections / microbiology*
  • Virulence Factors / genetics
  • Virulence Factors / isolation & purification*

Substances

  • DNA, Bacterial
  • Virulence Factors