Abstract
The hygromycin phosphotransferase gene was fused in-frame with the herpes simplex virus type 1 thymidine kinase gene. The resulting fusion gene (termed HyTK) confers hygromycin B resistance for dominant positive selection and ganciclovir sensitivity for negative selection and provides a means by which these selectable phenotypes may be expressed and regulated as a single genetic entity.
MeSH terms
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Animals
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Base Sequence
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Cell Line
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Cloning, Molecular / methods*
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Cytomegalovirus / genetics
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Ganciclovir / pharmacology
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Genes, Dominant*
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Mice
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Molecular Sequence Data
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Peptide Chain Initiation, Translational
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Phosphotransferases (Alcohol Group Acceptor)*
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Phosphotransferases / genetics*
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Plasmids
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Rats
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Restriction Mapping
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Selection, Genetic*
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Simplexvirus / enzymology
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Simplexvirus / genetics
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Thymidine Kinase / genetics*
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Transduction, Genetic
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Transfection
Substances
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Phosphotransferases
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Phosphotransferases (Alcohol Group Acceptor)
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hygromycin-B kinase
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Thymidine Kinase
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Ganciclovir