Receptor-operated Activation of Polymorphonuclear Leukocytes: Different Effects of NAP-1/IL-8 and fMet-Leu-Phe or C5a

Biochem Biophys Res Commun. 1991 May 15;176(3):972-8. doi: 10.1016/0006-291x(91)90377-j.

Abstract

We examined the production of PAF and LTB4 by PMN in response to NAP1/IL-8 alone, or after preincubation with GM-CSF, which has been shown to enhance PMN responsiveness and to prime PMN for production of those bioactive lipids. NAP-1/IL-8 does not induce the synthesis of PAF and LTB4 from endogenous phospholipid precursors, even after preincubation with GM-CSF. In addition and again in contrast to fMLP and C5a, NAP-1/IL-8 fails to induce an enhanced oxidative burst in GM-CSF primed PMN. Exogenously added PAF or LTB4 can mimic the priming effect of GM-CSF for an enhanced oxidative burst in response to all examined chemotactic peptides including NAP1/IL-8. Our data reveal a possible autocrine role of PAF and LTB4 in the enhanced responsiveness of GM-CSF primed PMN towards fMLP or C5a, but not NAP-1/IL-8.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arachidonate 5-Lipoxygenase / blood
  • Complement C5a / metabolism
  • Complement C5a / pharmacology*
  • Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology
  • Humans
  • Interleukin-8 / metabolism
  • Interleukin-8 / pharmacology*
  • Leukotriene B4 / pharmacology
  • N-Formylmethionine Leucyl-Phenylalanine / metabolism
  • N-Formylmethionine Leucyl-Phenylalanine / pharmacology*
  • Neutrophils / drug effects
  • Neutrophils / physiology*
  • Platelet Activating Factor / biosynthesis
  • Receptors, Formyl Peptide
  • Receptors, Immunologic / physiology*
  • Superoxides / blood

Substances

  • Interleukin-8
  • Platelet Activating Factor
  • Receptors, Formyl Peptide
  • Receptors, Immunologic
  • Superoxides
  • Leukotriene B4
  • N-Formylmethionine Leucyl-Phenylalanine
  • Complement C5a
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • Arachidonate 5-Lipoxygenase