Precise Characterization of Human Histones in the H2A Gene Family by Top Down Mass Spectrometry

J Proteome Res. 2006 Feb;5(2):248-53. doi: 10.1021/pr050269n.

Abstract

Top Down analysis revealed that at least fourteen genes encoding histone H2A are coexpressed in HeLa cells. Characterization of these species revealed that all except H2A.Z and H2A.F/Z were alpha-N-acetylated, H2A.O and H2A.C,D,I,N,P were the most abundant, and those exceeding approximately 10% abundance lacked post-translational modifications. This unequivocal identification of H2A forms illustrates the advantages of Top Down Mass Spectrometry and provides a global perspective of H2A regulation through the cell cycle.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation
  • Amino Acid Sequence
  • Chromatography, Affinity
  • HeLa Cells
  • Histones / chemistry*
  • Humans
  • Mass Spectrometry*
  • Molecular Sequence Data
  • Protein Isoforms / chemistry

Substances

  • Histones
  • Protein Isoforms