CT domain of CCN2/CTGF directly interacts with fibronectin and enhances cell adhesion of chondrocytes through integrin alpha5beta1

FEBS Lett. 2006 Feb 20;580(5):1376-82. doi: 10.1016/j.febslet.2006.01.061. Epub 2006 Jan 26.


Searching for CCN family protein 2/connective tissue growth factor (CCN2/CTGF) interactive proteins by yeast-two-hybrid screening, we identified fibronectin 1 gene product as a major binding partner of CCN2/CTGF in the chondrosarcoma-derived chondrocytic cell line HCS-2/8. Only the CT domain of CCN2/CTGF bound directly to fibronectin (FN). CCN2/CTGF and its CT domain enhanced the adhesion of HCS-2/8 cells to FN in a dose-dependent manner. The CCN2/CTGF-enhancing effect on cell adhesion to FN was abolished by a blocking antibody against alpha5beta1 integrin (alpha5beta1), but not by one against anti-alphavbeta3 integrin. These findings suggest for the first time that CCN2/CTGF enhances chondrocyte adhesion to FN through direct interaction of its C-terminal CT domain with FN, and that alpha5beta1 is involved in this adhesion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Cell Adhesion*
  • Cell Line
  • Chondrocytes / cytology*
  • Connective Tissue Growth Factor
  • Fibronectins / metabolism*
  • Humans
  • Immediate-Early Proteins / metabolism*
  • Integrin alpha5beta1 / metabolism*
  • Intercellular Signaling Peptides and Proteins / metabolism*
  • Membrane Proteins / analysis
  • Protein Binding
  • Two-Hybrid System Techniques


  • CCN2 protein, human
  • Fibronectins
  • Immediate-Early Proteins
  • Integrin alpha5beta1
  • Intercellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Connective Tissue Growth Factor