Abstract
In a hamster model of N-methyl-N-benzylnitrosamine (MBN)-induced oral carcinogenesis, the incidence of buccal pouch (HBP) carcinomas in MBN-treated hamsters (17.8+/-7.5) was significantly higher than MBN-treated hamsters given tea (10.8+/-3.9) (P<0.05). Amyloid precursor protein (APP) expression was also significantly increased in MBN-induced HBP carcinomas but was significantly reduced by tea intake (P<0.0001). Furthermore, APP expression and secretion by OECM-1 oral squamous cell carcinoma cells was inhibited by a major polyphenolic ingredient of green tea, (-)-epigallocatechin gallate, in a dose-dependent manner. Thus, APP might promote oral carcinogenesis, whereas green tea ingredients might diminish it by down-regulating APP.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amyloid beta-Protein Precursor / analysis
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Amyloid beta-Protein Precursor / genetics*
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Animals
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Blotting, Western
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Carcinoma, Squamous Cell / drug therapy*
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Carcinoma, Squamous Cell / genetics
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Carcinoma, Squamous Cell / pathology
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Catechin / analogs & derivatives
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Catechin / pharmacology
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Cell Line, Tumor
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Cricetinae
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Disease Models, Animal
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Dose-Response Relationship, Drug
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Gene Expression Regulation, Neoplastic / drug effects
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Humans
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Immunohistochemistry
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Male
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Mesocricetus
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Mouth Mucosa / drug effects
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Mouth Mucosa / metabolism
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Mouth Mucosa / pathology
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Mouth Neoplasms / drug therapy*
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Mouth Neoplasms / genetics
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Mouth Neoplasms / pathology
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Phytotherapy
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Plant Preparations / chemistry
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Plant Preparations / pharmacology*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Tea / chemistry*
Substances
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Amyloid beta-Protein Precursor
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Plant Preparations
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RNA, Messenger
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Tea
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Catechin
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epigallocatechin gallate