Expression of alkaline phosphatase in murine lymphoma cells

Biochem Biophys Res Commun. 1991 May 31;177(1):125-33. doi: 10.1016/0006-291x(91)91957-e.

Abstract

Alkaline phosphatase (ALP) was secreted and expressed at the cell surface of the lymphoma A/63-2 cell line but not on another clone A/63-1 deriving from a single thymoma (A/63) induced by a wild-type Abelson-Moloney viral complex. The enzyme was heat-sensitive and strongly inhibited by L-p-bromotetramisole and L-homoarginine but not by L-phenylalanine. All these data indicated that this enzyme was most likely identical to the L/B/K ALP isoenzyme. Southern blot analysis showed that neither amplification nor polymorphism were responsible for the high expression of the ALP gene observed in A/63-2 cells. On the opposite, the mRNA transcripts of ALP were only detected in A/63-2 cells indicating that a modulation of the ALP gene transcription occurred which could be due to the insertion of the v-abl gene within or near the 5'-flanking region of the ALP promotor in A/63-2 cells. Butyrate strongly increased both the secretion and the expression of the enzyme on A/63-2 cell surface. This induction was strongly inhibited by cordycepin, an RNA biosynthesis inhibitor, and at a lesser degree by cycloheximide, a translation inhibitor suggesting that butyrate induction occurs both at the transcriptional and the translational level.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / genetics*
  • Alkaline Phosphatase / metabolism
  • Animals
  • Blotting, Southern
  • Cell Line
  • Cloning, Molecular
  • DNA, Neoplasm / genetics
  • DNA, Neoplasm / isolation & purification
  • Kinetics
  • Lymphoma / enzymology*
  • Lymphoma / genetics
  • Mice
  • Phosphatidylinositol Diacylglycerol-Lyase
  • Phosphoric Diester Hydrolases / metabolism
  • RNA, Neoplasm / genetics
  • RNA, Neoplasm / isolation & purification
  • Restriction Mapping
  • Thymoma / enzymology
  • Thymoma / genetics
  • Thymus Neoplasms / enzymology
  • Thymus Neoplasms / genetics
  • Transcription, Genetic

Substances

  • DNA, Neoplasm
  • RNA, Neoplasm
  • Alkaline Phosphatase
  • Phosphoric Diester Hydrolases
  • Phosphatidylinositol Diacylglycerol-Lyase