Nuclear matrix attachment regions and topoisomerase II binding and reaction sites in the vicinity of a chicken DNA replication origin

Biochem Biophys Res Commun. 1991 May 31;177(1):265-70. doi: 10.1016/0006-291x(91)91977-k.

Abstract

We have mapped nuclear matrix attachment regions (MARs), defined by their specific binding to nuclear matrices in vitro, and sites of topoisomerase II reaction, detected by DNA cleavage in vitro in the presence of the inhibitor VM-26, in the vicinity of the replication origin of the chicken alpha-globin gene domain. Two MARs are located close to the downstream end (in the direction of transcription) of a 3 kb fragment which includes the origin. These MARs contain sites for strong topoisomerase II binding and reaction. Our observations on this gene domain support two hypotheses concerning MARs in eukaryotic cells, namely that they are close to DNA replication origins and that they contain multiple topoisomerase II recognition sites.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Binding Sites
  • Cell Line
  • Chickens
  • DNA / drug effects
  • DNA / genetics
  • DNA / metabolism
  • DNA Replication*
  • DNA Topoisomerases, Type II / metabolism*
  • Erythroblasts
  • Genes
  • Globins / genetics*
  • Molecular Sequence Data
  • Nuclear Matrix / metabolism*
  • Restriction Mapping
  • Teniposide / pharmacology

Substances

  • Globins
  • DNA
  • Teniposide
  • DNA Topoisomerases, Type II