Determination of the Degree of Acetylation of Chitosan by UV Spectrophotometry Using Dual Standards

Carbohydr Res. 2006 May 1;341(6):782-5. doi: 10.1016/j.carres.2006.01.008. Epub 2006 Feb 7.


Determination of the degree of acetylation of chitosan by UV spectrophotometry using dual standards is investigated. The UV absorbance of a pure chitosan solution is contributed additively by the N-acetylglucosamine and glucosamine residues; the absorbance divided by the total molar concentration of the residues (A/c(t)) is linearly related to the degree of acetylation (DA). Using acetyl glucosamine and glucosamine hydrochloride as standards in 0.1M hydrochloric acid solution, the equation obtained by linear regression is A/c(t)=3.3615 DA+0.0218, R(2)=0.9958. The DA of the analytical sample (m milligram of chitosan in V liters solution) can be calculated by.

MeSH terms

  • Acetylation
  • Animals
  • Brachyura
  • Chitosan / chemistry*
  • Reference Standards
  • Spectrophotometry, Ultraviolet / methods*


  • Chitosan