Knockdown of Stat3 in C17.2 neural stem cells facilitates the generation of neurons: a possibility of transplantation with a low level of oncogene

Neuroreport. 2006 Feb 27;17(3):235-8. doi: 10.1097/01.wnr.0000199470.29357.8f.


This study investigates the role of a low level of Stat3 in the C17.2 neural stem cells, which are popular stem cell candidates for transplantation research. The results reveal that C17.2 neural stem cells will undergo increased differentiation into neurons without generating glia after knockdown of Stat3 expression via an interfering RNA expression plasmid. As constitutively activated Stat3 is considered to be an oncogene, this study raises the possibility of stem cell transplantation with a low level of Stat3 to reduce the oncogenesis and facilitate the generation of neurons.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Differentiation / physiology*
  • Cell Line
  • Gene Expression Regulation / physiology
  • Green Fluorescent Proteins / metabolism
  • Humans
  • Immunohistochemistry / methods
  • Intermediate Filament Proteins / metabolism
  • Nerve Tissue Proteins / metabolism
  • Nestin
  • Neurons / cytology*
  • Neurons / physiology
  • RNA Interference
  • RNA, Messenger / biosynthesis
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • STAT3 Transcription Factor / genetics
  • STAT3 Transcription Factor / metabolism*
  • Stem Cells / physiology*
  • Transfection / methods
  • Tubulin / metabolism


  • Intermediate Filament Proteins
  • NES protein, human
  • Nerve Tissue Proteins
  • Nestin
  • RNA, Messenger
  • STAT3 Transcription Factor
  • Tubulin
  • Green Fluorescent Proteins