Abstract
We have expressed the murine coronavirus haemagglutinin-esterase protein in a vaccinia virus/T7 RNA polymerase system. The levels of expression observed are significantly higher than those found in virus-infected cells. The expressed protein has both receptor-destroying (esterase) and receptor-binding (haemadsorption) activities. The use of this system will greatly facilitate analysis of the structure-function relationships of this protein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Cell Line
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Cloning, Molecular
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Esterases / genetics
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Esterases / isolation & purification
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Esterases / metabolism
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Glycoproteins / genetics
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HeLa Cells / physiology
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Hemadsorption
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Hemagglutinins, Viral / genetics*
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Hemagglutinins, Viral / isolation & purification
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Hemagglutinins, Viral / metabolism
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Humans
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Kinetics
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Molecular Sequence Data
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Molecular Weight
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Murine hepatitis virus / genetics*
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Murine hepatitis virus / immunology
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Murine hepatitis virus / metabolism
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Oligonucleotide Probes
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Vaccinia virus / genetics*
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Viral Fusion Proteins*
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Viral Proteins / genetics*
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Viral Proteins / isolation & purification
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Viral Proteins / metabolism
Substances
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Glycoproteins
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Hemagglutinins, Viral
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Oligonucleotide Probes
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Recombinant Proteins
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Viral Fusion Proteins
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Viral Proteins
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hemagglutinin esterase
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Esterases
Associated data
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GENBANK/D00764
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GENBANK/M59337
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GENBANK/M59338
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GENBANK/M59339
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GENBANK/M59340
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GENBANK/M59341
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GENBANK/M59342
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GENBANK/M59343
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GENBANK/M59344
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GENBANK/M80795