High level transient expression of the murine coronavirus haemagglutinin-esterase

J Gen Virol. 1991 Jun;72 ( Pt 6):1309-15. doi: 10.1099/0022-1317-72-6-1309.

Abstract

We have expressed the murine coronavirus haemagglutinin-esterase protein in a vaccinia virus/T7 RNA polymerase system. The levels of expression observed are significantly higher than those found in virus-infected cells. The expressed protein has both receptor-destroying (esterase) and receptor-binding (haemadsorption) activities. The use of this system will greatly facilitate analysis of the structure-function relationships of this protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • Esterases / genetics
  • Esterases / isolation & purification
  • Esterases / metabolism
  • Glycoproteins / genetics
  • HeLa Cells / physiology
  • Hemadsorption
  • Hemagglutinins, Viral / genetics*
  • Hemagglutinins, Viral / isolation & purification
  • Hemagglutinins, Viral / metabolism
  • Humans
  • Kinetics
  • Molecular Sequence Data
  • Molecular Weight
  • Murine hepatitis virus / genetics*
  • Murine hepatitis virus / immunology
  • Murine hepatitis virus / metabolism
  • Oligonucleotide Probes
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Vaccinia virus / genetics*
  • Viral Fusion Proteins*
  • Viral Proteins / genetics*
  • Viral Proteins / isolation & purification
  • Viral Proteins / metabolism

Substances

  • Glycoproteins
  • Hemagglutinins, Viral
  • Oligonucleotide Probes
  • Recombinant Proteins
  • Viral Fusion Proteins
  • Viral Proteins
  • hemagglutinin esterase
  • Esterases

Associated data

  • GENBANK/D00764
  • GENBANK/M59337
  • GENBANK/M59338
  • GENBANK/M59339
  • GENBANK/M59340
  • GENBANK/M59341
  • GENBANK/M59342
  • GENBANK/M59343
  • GENBANK/M59344
  • GENBANK/M80795