Transmission electron microscopy and autofluorescence findings in the cornea of diabetic rats treated with aminoguanidine

Can J Ophthalmol. 2006 Feb;41(1):60-6. doi: 10.1016/s0008-4182(06)80068-2.

Abstract

Background: The accumulation of advanced glycation end products (AGEs) has been implicated in the pathogenesis of diabetic keratopathy. The present study was aimed to understand if aminoguanidine (AG), an AGE inhibitor, was protective against the development of corneal complications in a diabetic rat model.

Methods: Wistar rats were divided into three experimental groups: control, diabetic, and AG-treated diabetic. Diabetes was induced in rats via a single intraperitoneal injection (60 mg/kg) of streptozocin (STZ) and AG was administered in drinking water at a dose of 1 g/L. All animals were sacrificed at the end of 10 weeks and corneas from diabetic and nondiabetic rats were analyzed via transmission electron microscopy (TEM). Corneal autofluorescence measurements were also performed in all experimental groups.

Results: Electron microscopic evaluation revealed that aminoguanidine treatment in diabetic rats prevented the formation of intracellular spaces between neighbouring cells in the superficial corneal epithelium. Hyperglycemia-induced degeneration of intracellular organelles and formation of cytoplasmic vacuoles in the corneal stroma was also prevented with the treatment of AG. Corneal autofluorescence detected in the diabetic group (5.98 +/- 2.17 Fi/mg protein) was found to be significantly greater than the control (3.92 +/- 0.56 Fi/mg protein) and the AG-treated diabetic group (4.18 +/- 0.59 Fi/mg protein) (p < 0.05).

Interpretation: The presented data provide evidence that AG is preventive against corneal alterations in experimental diabetes.

MeSH terms

  • Animals
  • Cornea / ultrastructure*
  • Corneal Diseases / etiology
  • Corneal Diseases / pathology*
  • Diabetes Mellitus, Experimental / complications
  • Diabetes Mellitus, Experimental / drug therapy
  • Diabetes Mellitus, Experimental / pathology*
  • Disease Models, Animal
  • Enzyme Inhibitors / therapeutic use*
  • Guanidines / therapeutic use*
  • Male
  • Microscopy, Electron, Transmission / methods*
  • Nitric Oxide Synthase / antagonists & inhibitors
  • Rats
  • Rats, Wistar
  • Spectrometry, Fluorescence / methods
  • Streptozocin / toxicity

Substances

  • Enzyme Inhibitors
  • Guanidines
  • Streptozocin
  • Nitric Oxide Synthase
  • pimagedine