Increased expression of CCAAT/enhancer binding protein beta correlates with prognosis in glioma patients

Oncol Rep. 2006 Mar;15(3):595-601.


C/EBP beta (CCAAT/enhancer binding protein beta) is a transcriptional factor that belongs to the basic region-leucine zipper class DNA-binding proteins and plays a role in cell differentiation and inflammatory reactions. Although high tissue levels of inflammatory cytokines, such as interleukin (IL)-6, IL-8 and transforming growth factor-beta, have been observed in glioma patients, the mechanisms underlying this phenomenon remain to be elucidated. C/EBP beta induces a variety of cytokines and thus may play a role in the pathogenesis of glioma. In this study, we investigated the relationship between C/EBP beta expression, tumor histology, and prognosis in glioma. The expression of C/EBP beta mRNA was examined with quantitative real-time PCR and protein expression was examined with immunohistochemical techniques in 47 glioma tissue samples. Expression of C/EBP beta mRNA and protein was markedly increased in high grade glioma compared with low grade glioma. Patients whose expression of C/EBP beta mRNA and protein in tumor tissue was lower survived longer than those whose expressions were higher. In vitro, C/EBP beta siRNA inhibited glioma cell proliferation and invasion. Moreover, IL-8 production by glioma cells was inhibited by C/EBP beta siRNA transfection. These data suggest that increased expression of C/EBP beta may contribute to the promotion of tumor invasiveness and progression. The data imply that the comparison of C/EBP beta expression could be a prognostic marker for patients with glioma.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Astrocytoma / genetics
  • Astrocytoma / metabolism
  • Astrocytoma / pathology
  • Brain Neoplasms / genetics
  • Brain Neoplasms / metabolism
  • Brain Neoplasms / pathology*
  • CCAAT-Enhancer-Binding Protein-beta / analysis
  • CCAAT-Enhancer-Binding Protein-beta / genetics*
  • Cell Line, Tumor
  • Cell Movement
  • Cell Proliferation
  • Child
  • Female
  • Gene Expression
  • Glioblastoma / genetics
  • Glioblastoma / metabolism
  • Glioblastoma / pathology
  • Glioma / genetics
  • Glioma / metabolism
  • Glioma / pathology*
  • Humans
  • Immunohistochemistry
  • Interleukin-8 / metabolism
  • Male
  • Middle Aged
  • Prognosis
  • RNA Interference / physiology
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • RNA, Small Interfering / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Survival Analysis
  • Transfection


  • CCAAT-Enhancer-Binding Protein-beta
  • Interleukin-8
  • RNA, Messenger
  • RNA, Small Interfering