Antibody Response to Four Secretory Proteins From Mycobacterium Tuberculosis and Their Complex Antigen in TB Patients

Int J Tuberc Lung Dis. 2005 Dec;9(12):1327-34.


Objective: To evaluate antibody responses to a panel of four protein antigens secreted from Mycobacterium tuberculosis, CFP-21, ESAT-6, MPT-63 and MPT-64, and their complex antigen.

Design: Seventy-eight samples of serum and cerebral spinal fluid were tested by ELISA.

Results: Serum IgM antibody positive rates of protein antigens CFP-21, ESAT-6, MPT-63 and MPT-64 and their multi-antigen complex were respectively 34.6%, 60.3%, 52.6%, 78.2% and 96.2%; IgG antibody positive rates were respectively 46.2%, 64.1%, 93.6%, 57.7% and 84.6%. The specificity of the antibodies to the four proteins secreted from M. tuberculosis and its mixture antigen was 100%; sensitivity to the multi-antigen complex was higher than for the four single antigens. Among the IgM positive rates, the differences between the multi-antigen complex and CFP-21, ESAT-6, MPT-63 and MPT-64 were significant (P < 0.01); among the IgG positive rates, the differences between the multi-antigen complex and CFP-21, ESAT-6 and MPT-64 were significant (P < 0.01). Although the IgG positive rate for MPT-63 was higher than for the multi-antigen complex, the difference was not significantly different (P > 0.05).

Conclusion: These findings indicate that the multi-antigen complex composed of CFP-21, ESAT-6, MPT-63 and MPT-64 may be useful in the diagnosis of TB.

MeSH terms

  • Adult
  • Antigens, Bacterial*
  • Bacterial Proteins*
  • Case-Control Studies
  • Female
  • Humans
  • Immunoglobulin G / blood
  • Immunoglobulin M / blood
  • Male
  • Mycobacterium tuberculosis / immunology*
  • Sensitivity and Specificity
  • Tuberculosis / blood
  • Tuberculosis / immunology*


  • Antigens, Bacterial
  • Bacterial Proteins
  • CFP21 protein, Mycobacterium tuberculosis
  • ESAT-6 protein, Mycobacterium tuberculosis
  • Immunoglobulin G
  • Immunoglobulin M
  • MPB64 protein, Mycobacterium
  • MPT63 antigen, Mycobacterium tuberculosis