IL-4 and IL-2 upregulate the expression of antigen B7, the B cell counterstructure to T cell CD28: an amplification mechanism for T-B cell interactions

Int Immunol. 1991 Mar;3(3):229-35. doi: 10.1093/intimm/3.3.229.


We recently generated mAb 104 which is specific for the B cell activation antigen Ag B7. With this we studied the regulation of Ag B7 expression on normal tonsillar B lymphocytes as well as the activities of B7+ and B7- activated B cells. SAC and to a lesser extent anti-IgM antibody upregulated Ag B7 and this was further enhanced by IL-2 and most notably IL-4. Ag B7 was expressed on virtually all sIgG+ and sIgA+ B cells and approximately half of the sIgD+ and sIgM+ B cells. SAC-stimulated B7+ B cells proliferated and produced IgM, IgG and IgA in response to IL-2 and IgM and IgG in response to IL-4. SAC-stimulated B7- B cells proliferated and produced only IgM in response to IL-2 and IL-4. Considering that Ag B7 has recently been shown to be the counterstructure of the T cell CD28 and that CD28 triggering strongly enhances cytokine production by T cells, it is likely that the CD28/B7 interaction represents an important amplification phenomenon in T-B cell interaction leading to humoral immune responses. The preferential expression of Ag B7 on IgG and IgA committed cells suggests that CD28/B7 interaction may be more specific to secondary antibody responses provided by memory T and B cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Differentiation, T-Lymphocyte / analysis*
  • Antigens, Differentiation, T-Lymphocyte / physiology
  • Antigens, Surface / analysis*
  • B-Lymphocytes / immunology*
  • CD28 Antigens
  • Cell Communication*
  • Humans
  • Immunoglobulin A / analysis
  • Immunoglobulin G / analysis
  • Interleukin-2 / pharmacology*
  • Interleukin-4 / pharmacology*
  • Lymphocyte Activation
  • T-Lymphocytes / immunology*
  • Up-Regulation


  • Antigens, Differentiation, T-Lymphocyte
  • Antigens, Surface
  • CD28 Antigens
  • Immunoglobulin A
  • Immunoglobulin G
  • Interleukin-2
  • Interleukin-4